![]() ![]() "SarConfoCal: simultaneous sarcomere length and cytoplasmic calcium measurements for laser scanning confocal microscopy images. SarConfoCal is used to analyse scanlines and multi-channel (fluorescence and transmission) from Laser Scanning Confocal Microscopy (LSCM) images of myocytes, then it plots the fluorescence signal and the sarcomere length of each line versus time. Tested with ImageJ 1.50i but should works on some older versions. Others productions for ImageJ from the authors Magnitude = sqrt(10.142^2 1.255^2) = 10.2194Īnd with Matlab: Im = imread('circ.SarConfoCal - Plugin for ImageJ to measure Fluorescence and Sarcomere Length from Laser Scanning Confocal Microscopy (LSCM) Images SarConfoCal - Simultaneous Fluorescence and Sarcomere Length Measurements from Laser Scanning Confocal Microscopy (LSCM) ImagesĬôme PASQUALIN - François GANNIER (gannier at univ-tours dot fr), With ImageJ: Frequency = 10.24 pixels/cycle (25 cycles) The output is concordant with Matlab with adjustments nicely described by here and here. Also, it is necessary to click twice on the FFT window without moving the cursor to get the three output values. The credit goes to and The complex window has a slide bar at the bottom, which has to be drawn to the left. Whether this is correct, and whether there is an easier way to get this info without having to calculate on the side these values, or having to use several separate, and disjointed ( FFT and Complex) output windows, justifies keeping the post open. It gets interesting at the corners, where the PT tells us that the maximum number of cycles will be $\frac\right)=-61.78^\circ$ ![]() The direction, naturally can be at any angle around the clock face:Īnd if we select a darker pixel (as in this case), the value will consequently be low. The limit is 2 pixels/cycle for 128 cycles. Right, now the frequency has doubled to 4 cycles from side-to-side, which corresponds to 64 pixels/cycle, which (given enough patience and skepticism) can be proven by magnifying and counting the pixels: What happens if we move the cursor a tiny bit further away to the left along the x axis: ![]() The image is 256 x 256, and hence, the frequency is 256 pixels / cycle. This is the slowest frequency in the image, and corresponds to 1 cycle from side-to-side. First, placing the cursor very close to the bright dot in the center and along the x axis yields the following read-out: Picking up extreme positions of the cursor as it hovers over the FFT image or plot (yellow cross on the following images) makes it easier to interpret. Some playing with ImageJ has given me some preliminary intuition of what these numbers signify. I don't know if there is any additional information within this line to link to the actual sine (or cosine) wave at this particular point in the Fourier plot: Scale of the image was defined using Analyze/Set Scale. The angle is expressed inĭegrees, while the radius is expressed in pixels per cycle (p/c). ![]() Location is displayed in polar coordinates. If the mouse is over an active frequency domain (FFT) window, its Once an image is FFT-ed in ImageJ, placing the cursor over any points on the FFT plot results in an output of values at the bottom of the main menu, as in this example:Īccording to the documentation it simply outputs the location of the cursor: How to find pointwise readouts of the amplitude, frequency and phase of the DFT underpinning the FFT image? ![]()
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